2016

 

Genetically Encoded Voltage Indicators: Opportunities and Challenges.

 

Yang HH, St-Pierre F

The Journal of Neuroscience​

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DOI |​ PMID 27683896

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SUMMARY​

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We review the properties that determine indicator performance and applicability, discussing both recent progress and technical limitations. We then consider GEVI applications, highlighting studies that have already deployed GEVIs for biological discovery. We also examine which classes of biological questions GEVIs are primed to address and which ones are beyond their current capabilities

2017

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Fast two-photon imaging of subcellular voltage dynamics in neuronal tissue with genetically encoded indicators.

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Chamberland S, Yang HH, Pan MM, Evans SW, Guan S, Chavarha M, Yang Y, Salesse C, Wu H, Wu JC, Clandinin TR, Toth K, Lin MZ, St-Pierre F
 

Elife

 

DOI | PMID: 28749338

SUMMARY​

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We report ASAP2s, a novel voltage indicator with improved sensitivity. By imaging ASAP2s using random-access multi-photon microscopy, we demonstrate robust single-trial detection of action potentials in organotypic slice cultures. We also show that ASAP2s enables two-photon imaging of graded potentials in organotypic slice cultures and in Drosophila. These results demonstrate that the combination of ASAP2s and fast two-photon imaging methods enables detection of neural electrical activity with subcellular spatial resolution and millisecond-timescale precision.

2016

 

Subcellular Imaging of Voltage and Calcium Signals Reveals Neural Processing In Vivo.

Yang HH*, St-Pierre F*, Sun X, Ding X,

Lin MZ, Clandinin, TR

*co-first authors

Cell​

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DOI |​ PMID 27264607

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SUMMARY​

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We use in vivo, two-photon imaging of novel genetically encoded voltage indicators, as well as calcium imaging, to measure sensory stimulus-evoked signals in the Drosophila visual system with subcellular resolution. By imaging voltage and calcium signals to map information flow with subcellular resolution, we illuminate where and how critical computations arise.

2016

 

Subcellular Imaging of Voltage and Calcium Signals Reveals Neural Processing In Vivo.

Yang HH*, St-Pierre F*, Sun X, Ding X,

Lin MZ, Clandinin, TR

*co-first authors

Cell​

​

DOI |​ PMID 27264607

​

SUMMARY​

​

We use in vivo, two-photon imaging of novel genetically encoded voltage indicators, as well as calcium imaging, to measure sensory stimulus-evoked signals in the Drosophila visual system with subcellular resolution. By imaging voltage and calcium signals to map information flow with subcellular resolution, we illuminate where and how critical computations arise.