Choosing a GEVI Sensor

 

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For 1-photon imaging

JEDI-1P is our recommended sensor for detecting both action potentials and subthreshold voltage signals under 1P illumination. It is especially suitable when using a bright-to-dim sensor.

 

For 2-photon imaging

For action-potential detection under 2P illumination, we recommend JEDI-2P for most applications. Among our bright-to-dim sensors, JEDI-2P remains the preferred choice for spike detection because it is fast at suprathreshold voltages and has a larger dynamic range above approximately –45 mV than the other sensors.

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For subthreshold voltage detection

For experiments where detecting subthreshold voltage signals is important, we generally recommend JEDI3sub.

JEDI3sub and JEDI3hyp have broadly comparable performance. However, JEDI3sub is slightly brighter and can provide better spike detection in some comparisons, making it the preferred choice for most subthreshold-imaging applications.

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JEDI3hyp may be particularly useful when signals below –80 mV are important. It produces larger responses to voltage steps below –80 mV, especially at temperatures of 33 °C or higher. This may make it useful for studying voltage dynamics in astrocytes, mitochondria, or plants, where resting membrane potentials can be below –80 mV.

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JEDI-2Psub has a dynamic range similar to JEDI3hyp, but it is approximately half as bright at resting membrane potentials and has slower depolarization and repolarization kinetics. For most applications, we therefore recommend JEDI3hyp rather than JEDI-2Psub.

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We no longer distribute JEDI-1P AAVs or plasmids directly. Please obtain JEDI-1P constructs through Addgene and package them as needed.

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