DISSEMINATION RESOURCES
We are currently making new versions of our tools. If your application would benefit from increased performance, please contact Dr. St-Pierre (stpierre@bcm.edu), copying the laboratory manager, Dr. Wafa Hassen (wafa.hassen@bcm.edu)
Genetically Encoded Voltage Indicators (GEVIs)
JEDI-2P
JEDI-2P Flies: To request access please email Dr. St-Pierre
(stpierre@bcm.edu), Xiaoyu Lu (xl77@rice.edu), and Tom Clandinin
JEDI-2P plasmids are available on Addgene here
JEDI-2P specifications:
Paper: Liu, Zhuohe, et al. "Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy." Cell 185.18 (2022): 3408-3425. DOI: 10.1016/j.cell.2022.07.013
JEDI-1P
JEDI-1P plasmids coming soon on Addgene
JEDI-1P specifications:
Paper: Lu, Xiaoyu, et al. "Detecting rapid pan-cortical voltage dynamics in vivo with a brighter and faster voltage indicator." bioRxiv (2022): 2022-08. DOI: 10.1101/2022.08.29.505018
ASAP2s
ASAP2s is obsolete and should be replaced by JEDI-2P (two-photon applications) or JEDI-1P (one-photon applications)
ASAP2s plasmids are available on Addgene here
Paper: Chamberland, Simon, et al. "Fast two-photon imaging of subcellular voltage dynamics in neuronal tissue with genetically encoded indicators." elife 6 (2017): e25690. DOI: 10.7554/eLife.25690
Fluorescent proteins
mGOLD
Yellow Fluorescent Protein
mGOLD Plasmids are available on Addgene here
mGOLD specifications:
Paper:Lee, Jihwan, et al. "Versatile phenotype-activated cell sorting." Science advances 6.43 (2020): eabb7438. DOI: 10.1126/sciadv.abb7438
Engineered genetic circuits
Equalizer
Gene dosage circuits for homogeneous cell-to-cell expression
Equalizer plasmids are available on Addgene here
Paper: Yang, Jin, et al. "A synthetic circuit for buffering gene dosage variation between individual mammalian cells." Nature communications 12.1 (2021): 4132. DOI: 10.1038/s41467-021-23889-0
